FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66066693
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTAT3380930.5117450028867042TruSeq Adapter, Index 15 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1898050.28729302373285126No Hit
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT868490.13145655708845605No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA780310.11810943829139442No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA678700.1027295251481711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG486300.042.7211244-45
TATGCCG491700.035.6218648-49
CTCGTAT534150.032.57180444-45
GTATGCC655150.031.80229846-47
GCCGTCT661950.031.5012850-51
CGTATGC598600.029.17682546-47
TGTCGAG216350.028.0772675
ATGCCGT750350.028.02718248-49
AGTCCAA780050.027.09539634-35
CTGCTTG808200.026.39877558-59
GCTTGAA837800.025.17247260-61
GTCACAA855850.024.52831628-29
TCTCGTA838750.024.48643542-43
ATCTCGT707450.024.43533942-43
CACAAGT902250.023.54045930-31
TGCCGTC747400.023.49235250-51
CTGTCGA190000.023.3730814
CAGTCAC928600.022.60417226-27
AAATCTC765500.022.47958640-41
TGCTTGA791850.022.35628960-61
Produced by FastQC (version 0.11.5)