FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71938285
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTAT2290550.31840486605984564TruSeq Adapter, Index 15 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1601180.2225768935136555No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG904990.1258008861345527No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC826730.11492211692285964No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG755430.1050108436696816No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA727870.10117978208682624No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA721640.10031376199752329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG368700.038.1753444-45
GCCGTCT431500.032.7620550-51
GTATGCC448250.031.44812446-47
TATGCCG375200.030.26056748-49
ATGCCGT492500.028.91671648-49
TGTTACG181950.028.2313653
CGTATGC422400.026.98599646-47
GTTACGA198600.026.0799834
TTACGAC205400.025.2090385
TGTCGAG166850.024.1434825
CTCGTAT473300.024.09388244-45
TGTGTCG148600.022.7677153
TACGACT229600.022.6968176
TGCCGTC522700.022.0300650-51
GCTTGAA729850.019.86483660-61
GTCGAGG175850.019.3960066
AGTCCAA741750.019.22875634-35
CTGCTTG781350.018.749858-59
TTACGGA368750.018.4198958
GCGCTAT26650.018.4083351
Produced by FastQC (version 0.11.5)