FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74254592
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCCAAATCTCGTAT2402910.32360422908255965TruSeq Adapter, Index 15 (97% over 38bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA934500.12585080260086812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG396350.040.6147144-45
GTATGCC467750.034.6281646-47
GCCGTCT481250.033.42461450-51
ATGCCGT511050.031.72654748-49
TATGCCG394650.031.46293348-49
CGTATGC420500.029.57976246-47
CTCGTAT442850.027.93686744-45
AGTCCAA703500.023.24932934-35
TGCCGTC565200.022.3218250-51
GTCACAA761050.021.2115228-29
CACAAGT792400.020.82379730-31
CAAATCT772550.020.55070738-39
TCTCGTA780600.020.3269942-43
TGTTACG165350.020.3052583
CAAGTCC820100.020.02488132-33
TTACGAC177950.019.1343825
GCTTGAA863300.019.12059260-61
GTTACGA176950.019.108334
TTGAAAA861000.019.07237662-63
ACAAGTC694100.018.18210232-33
Produced by FastQC (version 0.11.5)