FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54962173
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2931380.5333449971128325No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG2107050.3833636635873185TruSeq Adapter, Index 7 (97% over 35bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA923360.1679991800906416No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA886490.16129093003655443No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG853030.15520310668939527No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA764980.1391829977319128No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT720750.1311356448734296No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT712110.12956365462479075No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT706270.12850110566043305No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA687030.12500051626415862No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT605170.11010663643156904No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG574150.10446275477499771No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA564440.10269608517843717No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC552970.10060919534604282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG297100.037.34821744-45
TATGCCG300300.036.55851746-47
CGTATGC343650.031.97824744-45
CTCGTAT344150.031.92856642-43
GCCGTCT347450.031.4526950-51
GTATGCC365150.030.48879446-47
ACCGCGT37450.029.3927826
TGTCGAG119450.028.9961875
TGTTACG104450.027.7110393
ATGCCGT399700.027.69124848-49
TGCCGTC399650.027.52823848-49
TATGATC382650.026.88610636-37
GATCTCG395250.026.50443340-41
GTTACGA111750.026.2391554
TGTGTCG100600.025.8943963
CCGCGTT44850.024.7547157
TTACGAC119900.024.4948985
GTCGAGG108200.024.3810316
GTTACGG335000.023.5674787
CTATGAT446550.023.52761336-37
Produced by FastQC (version 0.11.5)