FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005729532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66059529
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGATCTCGTAT4529190.6856225087526736TruSeq Adapter, Index 10 (97% over 37bp)
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1385330.20970933655915105No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1102300.1668646471881445No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGATCTCGTATG961770.14559141043830331TruSeq Adapter, Index 10 (97% over 36bp)
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG945410.14311485629877863No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA879240.1330981333518136No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC846840.1281934662295276No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG806490.12208533911890289No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG806040.12201721874220448No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA800660.12120280179412118No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT744820.11274981993892207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA704070.1065811413823432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)