FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66059529
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTCAGGGTGTAGATCT4675390.7077540622489149Illumina Single End PCR Primer 1 (97% over 37bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1937690.2933248282772346No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1125070.1703115382490844No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTCAGGGTGTAGATCTC997030.1509290203991615Illumina Single End PCR Primer 1 (97% over 36bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG674690.102133637677011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA647200.044.22487360-61
GCCGTAT650250.043.9360258-59
GGTCGCC720700.039.90120354-55
GTGGTCG736950.038.98571452-53
TCACCCG549450.036.694243
CTCGGTG792250.036.36210348-49
CGGTGGT796400.036.11083250-51
CGCCGTA659200.035.35249358-59
CCGTATC663950.035.0610860-61
GTAGATC843500.034.3082242-43
AGATCTC850450.034.20472744-45
CACCCGG595700.033.9687354
ATCTCGG867700.033.32729746-47
GTATCAT709700.032.8785262-63
TCTCGGT735450.031.83100148-49
TAGATCT756350.031.17634844-45
ATCATTA756950.030.86797564-65
TCGGTGG762300.030.74850550-51
GTGTAGT939650.030.57111228-29
GAGTGTA983450.029.3253826-27
Produced by FastQC (version 0.11.5)