FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121049366
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAACATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT23350581.9290129945827226No Hit
GGCCGGAACATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA6315430.5217235090681929No Hit
GGCCGGAACATGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1779220.1469830085685868No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA1211580.10008974355140364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA3991950.086.006614
GGAACAT4128100.083.1320345
CCGGAAC4140500.082.8972553
GCCGGAA4153350.082.637182
GGCCGGA4431450.077.568261
GAACATG3589600.077.0079656
CATGGCG3512250.071.125779
ACATGGC3607350.069.3388758
AACATTG995500.067.161087
GAACATT999400.066.875246
CATTGAA1038350.064.042019
AACATGG4046700.061.9374667
ACATTGA1076500.061.8915068
ACATGTA555700.048.418068
GCGATAA2823950.044.41908636-37
CGATAAC2828450.043.9019138-39
ATAACCG2830950.043.76913540-41
AGCGATA2840400.043.6519836-37
GATAACC2916300.042.94985638-39
ATCTCGA2932400.042.55663718-19
Produced by FastQC (version 0.11.5)