FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122675428
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATATCTCCGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5536470.4513104286866641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATATC998300.069.610311
ATATCTC1085800.063.9504363
AATATCT1203800.057.944492
ATCTCCG1198150.057.9039465
TATCTCC1297950.053.6710624
TCTCCGG1243650.048.4319656
TCCGGGC1391050.041.4931038
GCGATAA850500.037.55428736-37
TAACCGG906250.035.38204240-41
CTCCGGG1665150.034.8111467
TAAGACG946400.033.73586764-65
CGATAAC857900.033.7011538-39
ATAACCG862500.033.4571840-41
CGGAGTA960450.033.15042544-45
AGCGATA884600.032.6543536-37
GATAACC1003150.032.01486638-39
ATCTCGA1015600.031.79504818-19
AGTCGCC1032450.031.0380828-29
AGACGAC1069100.029.90367766-67
GTAATAA1082250.029.60482260-61
Produced by FastQC (version 0.11.5)