FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106222775
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAAGAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2649690.24944650523392933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG624100.054.363062
GGCTTAA649600.052.2640341
CTTAAGA734900.046.7520753
TTAAGAG822400.041.8386734
GCGATAA359550.037.91017536-37
TAAGAGA915950.037.7151035
CGATAAC364150.036.91958638-39
ATAACCG371650.036.1986240-41
TAACCGG388150.035.2344540-41
AGCGATA384150.034.9945836-37
AGAGGCG784950.034.4581689
TAAGACG403550.033.53163564-65
CGGAGTA423000.031.76558344-45
CGACCAA427750.031.2121470-71
GAGTCGC467050.028.72122228-29
AGTCGCC474000.028.69551828-29
AACCGGA472900.028.5532242-43
AGACGAC486150.028.08606766-67
ATCTCGA510400.027.06555718-19
GATAACC526800.026.25030738-39
Produced by FastQC (version 0.11.5)