FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106222775
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAAGAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2637880.24833469093610105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG640450.056.0225562
GGCTTAA660700.054.320521
CTTAAGA768300.047.582713
TTAAGAG840300.043.698774
TAAGAGA932400.039.3710025
GCGATAA382500.037.78205536-37
CGATAAC386700.036.5823338-39
ATAACCG390150.036.2467140-41
TAACCGG414500.034.9365240-41
AGCGATA406950.034.78852536-37
AGAGGCG826300.034.7489669
TAAGACG435300.033.09571564-65
CGGAGTA438150.031.842244-45
CGACCAA465250.030.28109270-71
AGTCGCC500350.028.56280728-29
GAGTCGC491900.028.45492428-29
AACCGGA509750.027.9053442-43
ATCTCGA525800.027.74351518-19
AGACGAC522350.027.71626566-67
GATAACC550050.026.45218838-39
Produced by FastQC (version 0.11.5)