FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78963209
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCACAGCACGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1827280.2314090350608725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCG367350.052.556199
GCACGGC434450.044.582058
AGCACGG437550.044.1142857
CAGCACG571950.036.307886
GCGATAA268050.036.21597736-37
CGATAAC270050.035.51577438-39
ATAACCG272650.035.17775340-41
TAACCGG280950.034.47659740-41
AGCGATA286050.033.61336536-37
ACGGCGT296250.032.518410-11
CGGAGTA300600.031.82046744-45
TAAGACG307300.031.65098664-65
GAGTCGC318200.030.30369428-29
AGTCGCC324550.029.9449628-29
CGACCAA323800.029.52924570-71
CGGCGTC347950.028.18496910-11
AACCACA891700.028.1166041
AACCGGA341950.028.05325742-43
AGACGAC358800.027.2135766-67
GTCGCCA360650.026.84076530-31
Produced by FastQC (version 0.11.5)