FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105957688
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGCCGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1349506512.736277333646614No Hit
AGGCCCGCCGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA50467624.762997471217002No Hit
AGGCCCGCCGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC18905601.7842593922962908No Hit
AGGCCCGCCGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC5664540.5346039638010976No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA5213940.4920775545800886No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG4174050.3939355490655855No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG3603140.34005460745802607No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT3549880.3350280727152144No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC3502630.3305687455166066No Hit
AGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACT3464630.3269824082986786No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT3388520.31979935236034973No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTG3356390.31676700986529643No Hit
TTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCC2993710.2825382524390302No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT2974800.28075357778663496No Hit
GACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATA2570390.2425864558313126No Hit
CGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA2509020.23679452122435893No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA2459990.23216720244027975No Hit
GCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGC2342060.2210372880163259No Hit
GGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAG2331600.22005010150844365No Hit
CAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCT2193910.20705529173116727No Hit
CGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTC2168530.2046599959787722No Hit
GTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAG2131030.20112084740844857No Hit
GCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCG2094530.19767607613333352No Hit
GTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCC2041020.19262594706671968No Hit
CAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAAC1973050.18621112231138906No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTAT1955100.18451704986239414TruSeq Adapter, Index 5 (97% over 36bp)
CAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCC1920650.1812657520424568No Hit
TGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAA1912050.18045410730366257No Hit
GCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAA1888030.17818716467275125No Hit
GCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCC1857480.17530393830412758No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG1786510.16860598166317106No Hit
GAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGT1781720.16815391441912172No Hit
TTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTC1736680.163903161042925No Hit
ATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTC1717420.16208545433720675No Hit
GTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGT1708470.1612407775450895No Hit
AAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCG1687270.15923997888666652No Hit
AGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGG1683790.1589115458993405No Hit
ATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTT1658930.15656532634045395No Hit
AAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATT1645230.15527235739609568No Hit
TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCC1610020.1519493328317998No Hit
TTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC1608700.15182475480212443No Hit
AATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATT1565360.14773444282778236No Hit
ATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGC1562670.14748056790367112No Hit
AGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGAT1558770.1471124964523575No Hit
CTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCT1554160.1466774171214457No Hit
TTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTT1553300.14659625264756626No Hit
TCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGC1512540.14274943409486246No Hit
AGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATT1507780.14230019816966938No Hit
AGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCT1488670.14049664805823245No Hit
AATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCT1486500.14029184932762973No Hit
CCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCC1478930.13957741320290037No Hit
TATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCT1465560.138315588765961No Hit
AACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATG1461510.13793336072036602No Hit
CGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGC1461330.1379163728072285No Hit
AAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGG1460960.1378814532080013No Hit
ACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCC1457860.13758888359285454No Hit
TCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCC1444710.13634782216086103No Hit
CGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTA1443720.1362543886386045No Hit
GACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCG1441540.1360486461350497No Hit
AGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTAT1435240.1354540691752353No Hit
GGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAA1429730.13493405027863575No Hit
ATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGA1417200.1337515027696716No Hit
ACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCA1411340.13319845181974904No Hit
GACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGC1408530.13293325162021277No Hit
TAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGA1393700.13153363633226878No Hit
CAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTAT1388820.13107307513165065No Hit
TTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGC1383310.13055305623505112No Hit
CGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCAT1376550.12991506571944075No Hit
TTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCA1373450.129622496104294No Hit
CTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCG1369400.12924026805869906No Hit
GTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGG1369390.12923932428574697No Hit
AGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATG1348410.1272592886322699No Hit
AATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCT1343140.1267619202865204No Hit
CTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGC1337310.1262117006554541No Hit
TAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGC1331220.12563694292763353No Hit
AGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAG1318040.12439305017678376No Hit
TAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATAT1317770.12436756830707744No Hit
AATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCAT1316800.12427602233072509No Hit
TGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGC1316670.12426375328234796No Hit
TGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCA1316440.12424204650444996No Hit
CGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGA1315210.12412596243134334No Hit
TGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAA1314400.12404951682222436No Hit
TATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTG1312690.12388813164741759No Hit
GCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGC1309010.12354082320104984No Hit
GTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAG1307090.12335961879424927No Hit
TGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGC1307060.12335678747539301No Hit
GTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAA1304000.1230679929520546No Hit
TCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTA1302120.12289056363706237No Hit
CCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGT1299700.12266217058265749No Hit
CAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTC1296040.12231674968219389No Hit
ATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATG1285500.12132201299069492No Hit
GGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAAT1284550.12123235456024672No Hit
GCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGT1271230.11997524898806776No Hit
TACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGC1270160.11987426528219454No Hit
GGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTG1265080.11939482862253468No Hit
ATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTA1251520.11811507249950566No Hit
TTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCA1251440.11810752231588896No Hit
GTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTT1248990.11787629794262783No Hit
ATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCG1244050.11741007410429717No Hit
TGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAA1227580.11585568005221103No Hit
TGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGT1224940.11560652399286026No Hit
GTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGC1222940.11541776940244299No Hit
TAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTG1218830.11502987871913552No Hit
CCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGC1218350.11498457761743537No Hit
CGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGG1210980.11428901695174777No Hit
CAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCA1208080.11401532279564272No Hit
GTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTG1207710.11398040319641554No Hit
AGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTC1200100.11326219197987784No Hit
AAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGG1197340.11300171064510203No Hit
GAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCAT1191850.11248357929440665No Hit
TTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATG1190700.11237504540491672No Hit
CAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAATT1189250.1122381983268642No Hit
AAGTAAGGGGCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTACATAC1188970.11221177268420579No Hit
TTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAAT1188450.11216269649069731No Hit
GGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACT1187680.11209002597338666No Hit
CAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGC1184870.1118248257738504No Hit
TGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAG1179740.11134067024943015No Hit
GAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGACGACCA1178320.11120665449023388No Hit
GGCTGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACAT1176860.11106886363922927No Hit
CGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCC1170340.110453523674469No Hit
ACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGT1168460.11027609435947677No Hit
TTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATA1166780.11011754050352628No Hit
GCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCG1166450.11008639599610742No Hit
GTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATT1165440.10999107492794671No Hit
TGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTAC1165060.10995521155576743No Hit
AATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGG1161330.10960318424463923No Hit
AATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATT1155310.10903503292748327No Hit
GAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAAT1147910.10833664094293942No Hit
TAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCA1141230.10770620061094575No Hit
CTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGG1138000.10740136194742189No Hit
TTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAA1132320.10686529891063687No Hit
TATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC1125080.10618200729332639No Hit
GGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGC1124100.1060895175440219No Hit
ATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACC1122170.10590736936426925No Hit
ATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTC1114220.10515706986736063No Hit
ACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGC1102970.10409532529626354No Hit
CGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTA1097760.10360361958822659No Hit
TAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAAT1085170.1024154094415499No Hit
GTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTT1083270.10223609258065351No Hit
TCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAA1083120.10222193598637222No Hit
CCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCG1081540.10207281985994258No Hit
CCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTA1078200.10175759969394575No Hit
AACGGCTGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGA1061260.10015884831311155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCCG22090350.094.822581
GGCCCGC22417400.093.343852
CGCCGTT5278250.093.2962656
CCGTTGA5274750.093.171658
CCGCCGT22396400.093.0704045
CCCGCCG22437700.092.9817054
CGTTGAA5295350.092.78059
CGCCGTG17175100.092.682956
CGTGGCG14617100.092.291969
GCCGTGG14657450.092.1198967
GCCCGCC22698650.092.109623
CCGTGGC14658800.092.013888
CCGTGTA2634450.091.0349668
GCCGTGT2675700.089.8409967
GGGCCCG239650.047.4743582
GTGGCGT14656600.046.49247710-11
GGCGTCC14715250.046.43808712-13
CGTCCAT14734650.046.37021614-15
TACGCTG2639350.046.34995312-13
GCGTCCA14719400.046.25477612-13
Produced by FastQC (version 0.11.5)