FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005729553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105957688
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGCCGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT96827419.138309057857134No Hit
AGGCCCGCCGTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA32140293.033313637421005No Hit
AGGCCCGCCGTGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC12721161.200588672716226No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA6238920.588812394623031No Hit
GACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATA4958550.4679745371567564No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG4833770.45619813826062344No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT4476220.4224535363587775No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC4349560.41049970814765224No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT4339660.4095653729250868No Hit
AGGCCCGCCGTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC4285970.4044982559453354No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG4108780.38777554300731815No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTG3761000.35495300727966056No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT3361870.3172841974430397No Hit
AAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCG3232290.30505478752990534No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA3049890.28784036888385106No Hit
AGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGG3003920.28350184462311034No Hit
GCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGC2802510.26449331359514No Hit
CAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCT2616610.24694857441585552No Hit
AGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACT2560480.24165117683579507No Hit
CGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCAT2546340.24031668188154504No Hit
AATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCT2504750.2363915301738181No Hit
CGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA2458830.23205772477783776No Hit
GACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCG2453220.23152826815171731No Hit
ATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATG2451590.23137443316052722No Hit
CGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTC2444430.23069869172683347No Hit
AATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCAT2376600.224297079792832No Hit
CTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGG2308200.21784167280056166No Hit
TTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCC2296620.2167487837220457No Hit
GGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAG2200950.207719707889436No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG2194240.20708643623858608No Hit
AGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAG2176850.20544521507490804No Hit
GCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCC2175120.2052819423541971No Hit
ATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCG2151570.20305935705203382No Hit
ATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTC2105950.1987538648446161No Hit
CAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGC2099080.19810549282653278No Hit
ACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCA2081410.19643784602019632No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2064850.19487495801154137Illumina Single End PCR Primer 1 (100% over 50bp)
TAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGC2046960.19318654820025896No Hit
GACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGC2028270.19142263655280964No Hit
GTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGT2012000.1898871179597652No Hit
CAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAAC1997120.1884827838070608No Hit
CTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAG1927050.18186976673179203No Hit
CAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCC1877020.17714807065250424No Hit
ACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATAC1871150.17659407592962956No Hit
AAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAA1856190.17518219159330845No Hit
TGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGC1838940.17355418325095956No Hit
ACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGC1802360.17010186179222786No Hit
AAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTT1777130.16772072263411408No Hit
ATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTT1727540.1630405525647181No Hit
GTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGC1715570.16191085634107077No Hit
GCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCG1707090.1611105368777016No Hit
TAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCA1704670.1608821438232967No Hit
GTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTC1695690.1600346357123232No Hit
ATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGC1678580.15841984019130353No Hit
AGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATT1677700.15833678817151994No Hit
CAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAATT1617590.15266376895652914No Hit
CCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACG1609900.15193800755637477No Hit
GTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCC1601970.15118959560537032No Hit
GCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAA1586880.1497654422206721No Hit
GTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAG1569980.14817046593164623No Hit
CAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCA1562770.147490005633192No Hit
GTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTT1552650.14653490740568065No Hit
AATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAA1551830.14645751802360957No Hit
TTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTC1535550.14492105565761307No Hit
GGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCC1531300.1445199521529764No Hit
ATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAG1509090.1424238324263927No Hit
GCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGC1479300.13961233280212756No Hit
CGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGC1474020.13911402068342602No Hit
TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCC1470610.13879219410676458No Hit
GAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGA1462850.1380598262959456No Hit
CTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAA1448930.13674609434664145No Hit
AGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCT1438530.1357645704764717No Hit
ATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTC1434900.13542198089486437No Hit
AGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGAT1424050.13439798724185073No Hit
GGCTGCGGACGACCAGGGCGAGCGCCAGAACGTTTTTTACCTTTAGACAT1420270.1340412410659621No Hit
GTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTG1397350.1318781134597803No Hit
AGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTAT1391430.1313193998721452No Hit
GCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGT1387030.1309041397732272No Hit
GGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAA1376150.12987731480135734No Hit
GGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGT1354260.1278113958092404No Hit
CAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTAT1353360.1277264562435526No Hit
AAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGG1318410.12442796977601098No Hit
TTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGC1310760.12370598346766494No Hit
GAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGT1303560.1230264669421628No Hit
CCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGC1294650.1221855652418539No Hit
AATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCT1292670.12199869819734081No Hit
AATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATT1288540.12160891996812917No Hit
CTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGGCCCCT1275420.12037068985499193No Hit
CGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGA1270150.11987332150924243No Hit
TGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGG1261630.11906922695406491No Hit
TCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCG1259350.11885404672098923No Hit
GTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATG1250930.11805938989533256No Hit
TCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAA1250190.11798955069687816No Hit
CGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTA1245310.11752898949626005No Hit
AGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGCATG1241370.11715714295313805No Hit
AATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATT1240170.1170438901988877No Hit
CCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAG1232610.11633039784711044No Hit
CAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTT1231670.11624168318961432No Hit
TGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGG1229620.11604820973443664No Hit
TATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTC1221010.11523562122269032No Hit
TGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAA1219660.11510821187415868No Hit
AAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATT1219650.1151072681012066No Hit
AACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATG1215570.11472220873675537No Hit
GAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCA1213050.11448437795282963No Hit
ATACGCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAG1210970.11428807317879568No Hit
CCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGGCGCC1209270.114127631776941No Hit
CTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGC1195260.11280540587106808No Hit
AGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAA1191050.11240807745823975No Hit
TGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGC1184060.11174838016473142No Hit
GCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGT1178850.11125667445669445No Hit
GCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTC1174400.11083669549301606No Hit
GAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAAT1172490.11065643485916754No Hit
AATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGG1161460.10961545329301635No Hit
TTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCA1158550.10934081536395925No Hit
CTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCT1157730.10926342598188815No Hit
GCCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAA1150760.10860561623428401No Hit
AACCGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGA1149630.10849896989069827No Hit
ATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTA1148140.10835834772083738No Hit
TATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCT1139130.10750800829100764No Hit
GTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAG1138550.10745326945978662No Hit
GCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGT1134830.10710218592161053No Hit
CTAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTT1129240.10657461684139427No Hit
CTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCGG1124530.10613009978096162No Hit
ATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATTA1123190.10600363420538204No Hit
GTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAATTTTTGAC1111670.10491640776457863No Hit
TTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGCGTATGCCGC1101190.10392733371079217No Hit
CGGAGTAGTTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGC1099090.10372914139085405No Hit
AGCGCCAGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACG1095930.10343090913799478No Hit
AGAACGTTTTTTACCTTTAGACATTACATCACTCCTTCTGCACGTAATTT1093260.10317892175978774No Hit
GCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGT1093010.10315532743598557No Hit
GCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGAGC1089350.102809906535522No Hit
TTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCA1084610.10236255815623309No Hit
CTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCC1081970.1021134020968823No Hit
TTGAAATGGTAATAAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGG1076220.10157073264943266No Hit
GCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTG1075310.10148484931079282No Hit
GGCCCGCCGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA1074030.10136404637292576No Hit
CAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTC1066080.10061374687601715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)