FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116927967
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT8024900.686311427958035No Hit
CATAATGGCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2170800.18565276175544898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA1543250.071.5299155
TAATGGC1564050.070.8406753
CATAATG1579200.070.5643161
ATAATGG1592300.069.7992862
TGGCGAG1306150.068.346376
AATGGCG1743000.063.4604954
GCGAGGC1279000.063.013388
GGCGAGG1447550.055.794017
CGAGGCG1641950.049.064159
GCGATGA484100.044.201818
CGATGAA491950.043.592979
GCGATAA951000.042.6899936-37
TGGCGAT508350.042.3171166
CGATAAC954700.042.2661738-39
ATAACCG961250.042.03046840-41
GCGAGTA211700.041.861498
AGCGATA972650.041.5128236-37
TAACCGG1009400.040.31947340-41
CGGAGTA1014300.039.73913244-45
TAAGACG1036050.039.1610564-65
Produced by FastQC (version 0.11.5)