FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116927967
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT6982840.5971916025872578No Hit
CATAATGGCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2091540.17887422946470968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCGA1467850.069.539475
TAATGGC1490650.069.093073
CATAATG1499600.069.026621
ATAATGG1517000.068.0885852
TGGCGAG1230800.065.647786
GCGAGGC1209450.060.1486748
AATGGCG1715750.059.694134
GGCGAGG1366900.053.3235787
CGAGGCG1457800.050.071179
CGATGAA477900.045.0335779
GCGATGA486200.044.333178
TGGCGAT503000.042.983846
GCGATAA884950.041.82917436-37
CGATAAC885350.041.16500538-39
ATAACCG899300.040.6159940-41
AGCGATA913750.040.0637836-37
TAACCGG948700.038.9966640-41
GCGAGTA210150.038.889748
CGGAGTA954950.038.30586244-45
TAAGACG982850.037.54354564-65
Produced by FastQC (version 0.11.5)