FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95353744
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAATTGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3609780.3785672013046493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTGA764850.054.927414
AATTGAG777550.053.8907435
CTTAATT853500.049.5753062
TCTTAAT884100.047.920511
ATTGAGG827850.044.8221176
GAGGGCG831650.042.3918539
TTAATTG1042600.040.7457353
GCGATAA440900.040.3631136-37
CGATAAC441450.039.93663438-39
ATAACCG446200.039.4755440-41
TTGAGGG912600.038.890257
TAACCGG464800.038.30969240-41
AGCGATA461650.038.199136-37
TAAGACG489050.036.4436764-65
CGGAGTA497000.035.5790244-45
CGACCAA504700.034.8597970-71
GAGTCGC526850.033.49507528-29
AACCGGA532400.033.21727842-43
AGTCGCC541650.032.93060728-29
AGACGAC550300.032.4436666-67
Produced by FastQC (version 0.11.5)