FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93922526
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCCCCTAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT4305270.45838524402548547No Hit
GAATCCCCCTATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1396330.14866827580851052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG584600.073.9598169
CCCCCTA915900.066.771215
AATCCCC1024250.060.575292
GAATCCC1044200.059.551581
CCCCTAG813150.058.5337226
ATCCCCC1090750.056.3258483
CCCTAGG812900.053.6091237
CCTAGGC936600.046.640338
GCGATAA550300.041.05494736-37
TCCCCCT1508600.041.001264
CCCCTAT353950.039.2469866
CGATAAC557450.038.99747538-39
ATAACCG565100.038.60037640-41
TAACCGG597150.038.043740-41
AGCGATA571250.037.9904436-37
CGGAGTA608200.036.9072444-45
TAAGACG643550.035.11175564-65
AGTCGCC648550.034.8851728-29
TAGGCGT626050.034.77699310-11
GAGTCGC637050.034.09476528-29
Produced by FastQC (version 0.11.5)