FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107259685
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCGCGACGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT16288211.518577087001514No Hit
GAGTCGCGACGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3219410.3001509840346818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGCG2482100.085.720012
CGCGACG2592400.082.6177755
TCGCGAC2585000.082.016764
GTCGCGA2597750.081.863943
GCGACGG2353150.077.451546
CGACGGG2234450.076.413717
ACGGGCG2376450.072.284899
GACGGGC2528200.067.954138
GCGACGT529300.061.7477846
TCCGATC304850.058.0917936
TTCCGAT317050.055.9313285
ACGGTAC220100.051.893239
CGACGGT232850.049.5606277
ACGTGAA666150.049.1133889
CGACGTG669750.049.0327457
GACGGTA237500.048.3303348
GACGTGA708950.046.0469328
GAGTCGC4699200.045.6609231
CCGATCT382450.045.609267
CGATCTA101200.040.4044728
Produced by FastQC (version 0.11.5)