FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106454510
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATATGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT11807761.1091836315812267No Hit
AGCCATATGAGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3019570.28364885621097685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGA1987200.081.526884
GCCATAT1996000.081.451962
ATATGAG1995900.081.125945
CCATATG2015500.080.601593
AGCCATA2104750.077.111631
TATGAGG1795600.072.809056
ATGAGGG2013850.060.6914637
GAGGGCG2061600.059.3654679
TATGAGT587200.053.492876
TGAGGGC2443200.050.2585038
GCGATAA1479100.042.2840236-37
CGATAAC1483900.041.33048638-39
ATAACCG1492700.041.083740-41
AGCGATA1507950.040.73804536-37
TAACCGG1579350.039.66117540-41
CGGAGTA1562150.039.26460644-45
ATCTCGA1594650.039.2182518-19
GATAACC1611700.038.8930138-39
AGTCGCC1594700.038.60801728-29
GAGTCGC1588450.038.04533828-29
Produced by FastQC (version 0.11.5)