FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116360034
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCTTCGATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT7742900.6654260688854732No Hit
GGTCCTTCGATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2518160.2164110746134708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT1422150.079.290365
TTCGATG1140800.076.525856
CGATTGA342900.073.647318
TCGATTG344650.073.49237
TCCTTCG1669500.067.642763
TCGATGG1152100.067.2177
CCTTCGA1681100.067.1038744
TTCGATT387500.065.671896
GGTCCTT1890250.060.1747971
CGATGGC1340800.057.825618
GATGGCG1460350.053.1991129
GTCCTTC2160800.052.701082
GCGATAA969700.041.5804736-37
CGATAAC975800.040.249938-39
GATTGAA640650.039.9007039
ATAACCG984950.039.87434840-41
AGCGATA995450.039.3478736-37
TAACCGG1047550.038.68848840-41
CGGAGTA1051450.037.85785344-45
TAAGACG1094550.036.72628464-65
Produced by FastQC (version 0.11.5)