FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116360034
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCCTTCGATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT8057910.6924980788506817No Hit
GGTCCTTCGATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2342800.20134060806479312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT1496350.078.582245
TTCGATG1236200.075.427766
CGATTGA336250.071.948498
TCGATTG340550.071.3426747
TCCTTCG1723750.068.2681963
TCGATGG1238800.067.8243567
CCTTCGA1740500.067.732324
TTCGATT378400.064.26386
GGTCCTT1919100.061.7461621
CGATGGC1431300.058.8014568
GATGGCG1559850.054.0058449
GTCCTTC2200600.053.9943432
GCGATAA1084050.040.92598336-37
CGATAAC1087950.039.10761638-39
ATAACCG1097250.038.7745940-41
AGCGATA1107400.038.45349536-37
TAACCGG1162200.038.20648240-41
GATTGAA644900.037.8018539
CGGAGTA1158100.037.268344-45
ATCTCGA1216500.036.66916718-19
Produced by FastQC (version 0.11.5)