FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121491924
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTCGATATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2389190.19665422369967572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA391750.078.0704964
TAGTCGA402300.075.990332
CGATATG366550.072.672716
TCGATAT439250.069.665225
GTAGTCG457850.067.00241
AGTCGAT462100.066.2979353
TATGGCG378650.066.153199
ATATGGC667650.037.9961788
GCGATAA339050.037.4886936-37
CGATAAC343900.036.6570338-39
GATATGG711850.035.7327657
ATAACCG354500.035.7151140-41
TAACCGG372500.034.35907740-41
AGCGATA371650.033.98941836-37
TAAGACG395250.032.2167164-65
CGGAGTA413400.030.47122844-45
CGACCAA420350.030.1803670-71
GAGTCGC452850.028.0728428-29
AACCGGA453750.028.04463842-43
AGACGAC462750.027.73805266-67
Produced by FastQC (version 0.11.5)