FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103603139
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACGAATAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT11288061.089548068615952No Hit
GTTGACGAATATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3919400.37830900084986807No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA1128980.10897160171951933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAATA1827850.088.3580865
GACGAAT1830650.088.224714
CGAATAG1419250.086.784786
TTGACGA1879000.086.3382
GTTGACG1907250.085.091451
TGACGAA1913250.084.475343
ATAGGCG1380850.081.400319
GAATAGG1461750.076.96887
CGAATAT506700.075.817596
AATAGGC1504250.074.880588
GAATATG843150.045.8901067
GCGATAA1315500.043.45699336-37
CGATAAC1315050.042.5629238-39
ATAACCG1326550.042.3872840-41
AATATGA912950.042.3248028
AGCGATA1333950.041.8892236-37
ATATGAA928650.041.517189
CGGAGTA1379250.041.03350444-45
TAACCGG1412800.040.67706740-41
TAGGCGT1389800.040.49304610-11
Produced by FastQC (version 0.11.5)