FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103603139
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACGAATAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18504761.786119627128286No Hit
GTTGACGAATATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA3349740.32332418036098304No Hit
TTGACGAATAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA1244490.12012087780467734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACG2615800.089.529471
GACGAAT2727550.085.813964
ACGAATA2745700.085.358915
TTGACGA2759200.084.841652
CGAATAG2382050.084.23156
TGACGAA2814500.083.31643
ATAGGCG2403800.079.655779
GAATAGG2434250.078.6593557
AATAGGC2455300.078.0256048
CGAATAT467050.072.7401056
GCGATAA2401700.042.8422336-37
GAATATG805600.042.606147
AGTCGCC2473150.041.22426228-29
ATCTCGA2502050.041.12039618-19
TAACCGG2504050.041.02361340-41
CGGAGTA2471600.041.00023744-45
AGGCGTC2512200.040.8328510-11
GATAACC2534950.040.54337738-39
CGAAGGA2544500.040.45122-23
GCGTCCA2558350.040.1452312-13
Produced by FastQC (version 0.11.5)