FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101054953
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18429601.8237206047683778No Hit
ATACTGTATCATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2324530.23002633032742095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGTA2675100.085.7916952
ATACTGT2698650.085.418671
TGTATCA2716900.083.9451755
ACTGTAT2748000.083.460183
GTATCAG2455400.082.5860446
CTGTATC2816650.081.335864
TATCAGG2395400.081.057497
TCAGGCG2467500.079.0739069
ATCAGGC2609600.074.763438
GTATCAT484950.051.822076
TATCATG509700.049.268347
GCGATAA2237600.044.44372636-37
CGATAAC2233900.043.46825838-39
ATAACCG2241300.043.3600840-41
TAACCGG2289550.043.29235540-41
AGCGATA2260900.043.10588536-37
TAAGACG2313750.042.84653564-65
CGGAGTA2296250.042.81716544-45
GATAACC2355650.042.0943238-39
AGTCGCC2335950.041.91808728-29
Produced by FastQC (version 0.11.5)