FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117994963
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACAAGTATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT31053202.631739458234332No Hit
TTAACAAGTATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2723330.2308005300192348No Hit
TAACAAGTATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA1721860.14592656806884205No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA1471500.12470871320159659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAA4145550.087.072331
TAACAAG4282750.084.045352
TATGGCG3879850.083.637779
CAAGTAT4342700.082.4349755
ACAAGTA4401500.081.713664
AAGTATG4080200.080.9361656
GTATGGC4000700.080.77718
AACAAGT4457100.080.739483
AGTATGG4017050.080.307177
GTATTGA503850.054.6935848
AGTATTG528850.052.852447
TATTGAA614100.045.0738379
GCGATAA4000200.043.86824436-37
TAACCGG4066800.042.9945840-41
ATCTCGA4064050.042.82317418-19
AAGTATT661200.042.626446
CGGAGTA4049800.042.5122644-45
GATAACC4134850.042.46825838-39
AGTCGCC4093850.042.173328-29
CGAAGGA4158250.041.94457222-23
Produced by FastQC (version 0.11.5)