FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113694985
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCACCCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT14592611.2834875698343247No Hit
TTCGCACCCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA4770450.4195831504793285No Hit
TTCGCACCCGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1667260.14664323144947863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAC2295450.090.975731
TCGCACC2439000.085.617122
CACCCGA2461300.084.7820055
ACCCGAG1998050.080.972926
ACCCGAT599150.078.484036
CGCACCC2672950.078.141933
GCACCCG2724150.076.708114
CCCGAGG2184400.066.183587
CCGATGA728500.064.5291068
CGAGGCG2280000.063.098259
CGATGAA750050.062.6288229
CCCGATG758200.062.164267
CCGAGGC2941500.049.10698
CGAGTAC369750.047.9997679
GCGATAA1670800.043.32576436-37
CGATAAC1673700.043.07687838-39
AGCGATA1687900.042.66734336-37
ATAACCG1693850.042.58086840-41
CGGAGTA1741050.041.16277744-45
GAGTCGC1760300.040.77809528-29
Produced by FastQC (version 0.11.5)