FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113694985
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCACCCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18518101.6287525786647494No Hit
TTCGCACCCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA4019430.3535274664929152No Hit
TTCGCACCCGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1360480.11966051097152615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAC2758650.092.0756451
TCGCACC2944950.085.982012
CACCCGA2973250.084.98735
ACCCGAG2565600.082.565116
CGCACCC3153600.080.30623
GCACCCG3231950.078.476934
ACCCGAT555150.074.627266
CCCGAGG2779600.071.060817
CGAGGCG2802050.070.633659
CCGATGA676900.060.901488
CGATGAA694150.059.475299
CCCGATG713300.058.360837
CCGAGGC3409600.057.967068
CGAGTAC344700.043.3398789
GCGATAA2302900.043.2095836-37
CGATAAC2301450.042.65872638-39
AGCGATA2322000.042.36431536-37
ATAACCG2320450.042.31914540-41
ATCTCGA2388500.041.6748218-19
AGTCGCC2356950.041.3917728-29
Produced by FastQC (version 0.11.5)