FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211938455
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTCGATATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9396870.44337730026389033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA1543950.082.59684
TAGTCGA1548250.082.4635242
GTAGTCG1602800.079.920371
TCGATAT1631650.078.250445
CGATATG1374200.077.857666
AGTCGAT1662650.076.822893
TATGGCG1383750.071.6768659
ATATGGC1810350.055.0673078
GATATGG1856450.053.6160667
CGATATT424300.049.3643536
GCGATAA1368150.038.71218536-37
TAACCGG1450050.036.5008240-41
CGATAAC1378200.036.24120338-39
ATAACCG1385450.036.04810340-41
AGCGATA1421100.035.2075936-37
CGGAGTA1490800.034.6862944-45
TAAGACG1521350.034.57852664-65
AGTCGCC1563050.033.48095728-29
ATCTCGA1632900.032.43852218-19
GAGTCGC1550100.031.94984428-29
Produced by FastQC (version 0.11.5)