FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110545109
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATCTAGAGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT12212221.1047273018655217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAG1527900.083.756893
TTATCTA1635300.078.468622
ATCTAGA1645600.077.815324
TCTAGAG1718000.074.526435
CTTATCT1756000.073.2400051
TAGAGGG1688400.072.2634057
CTAGAGG1805850.071.142856
GAGGGCG1905100.064.071239
AGAGGGC2231900.054.8577048
GCGATAA1394400.043.92231836-37
CGATAAC1392150.043.6026138-39
ATAACCG1407000.043.30779640-41
AGCGATA1416600.042.88363336-37
TAACCGG1456700.042.21821240-41
CGGAGTA1465450.041.63012744-45
TAAGACG1483150.041.14806464-65
AGTCGCC1515150.040.4771228-29
GAGTCGC1512500.040.24029528-29
ATCTCGA1546300.039.95619218-19
GATAACC1556650.039.5501838-39
Produced by FastQC (version 0.11.5)