FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105113944
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAGAATCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT14753291.4035521300580254No Hit
TGTAAGAATCATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA5358480.5097782269496043No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA1778940.16923920198446746No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT1236190.11760475850853813No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG1109230.10552643710143726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGA2724650.081.208461
TAAGAAT2830100.078.432943
TCAGGCG1962150.077.1893849
AAGAATC2921800.076.187524
GAATCAG2319250.072.7868046
AATCAGG2109350.071.99647
ATCAGGC2130850.071.1384668
GTAAGAA3172550.069.788922
AGAATCA3259100.068.509435
GAATCAT981750.055.505456
AATCATG1061450.051.422787
TCATGAA1223600.044.654879
GCGATAA1758700.043.42665536-37
CGATAAC1762100.042.99700538-39
ATAACCG1775750.042.7970340-41
AGCGATA1778100.042.63096636-37
CGGAGTA1813350.041.50444444-45
ATCTCGA1873800.040.9483918-19
ATCATGA1337000.040.8078
TAACCGG1878400.040.7149240-41
Produced by FastQC (version 0.11.5)