FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105113944
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAGAATCAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT25995422.4730705566523126No Hit
TGTAAGAATCATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA5184250.49320288086611996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGA3972750.087.091581
TAAGAAT4250050.081.5376053
AAGAATC4399200.078.773164
TCAGGCG3526650.077.910989
GAATCAG3795000.076.695076
GTAAGAA4566550.076.069972
AATCAGG3628250.075.787077
ATCAGGC3657250.075.204658
AGAATCA4850050.071.420265
GAATCAT991700.055.644786
AATCATG1093700.050.5160527
TCATGAA1222300.044.753529
ATCATGA1253000.043.7566228
GCGATAA3462050.041.57342536-37
AGGCGTC3484500.041.02836610-11
ATCTCGA3498000.040.84723318-19
AGTCGCC3488950.040.62065528-29
CGGAGTA3458950.040.40245444-45
CGAAGGA3557050.040.3468822-23
GCGTCCA3556250.040.25021412-13
Produced by FastQC (version 0.11.5)