FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175659225
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAACATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT18894051.0756081839709812No Hit
GGCCGGAACATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8312130.47319632658062794No Hit
GGCCGGAACATGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2820150.16054664934335217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA3661850.085.3405764
CCGGAAC3741300.083.498763
GCCGGAA3750800.083.253922
GGCCGGA3941250.079.380361
GGAACAT4231650.073.9706655
CATGGCG2799800.068.1298149
GAACATG3604050.064.079376
ACATGGC3433650.055.7494858
AACATTG1696050.049.031817
GAACATT1762600.047.2208486
ACATTGA1806150.045.987698
CATTGAA1816350.045.7189839
AACATGG4316600.044.57387
GCGATAA2157050.044.29769536-37
CGATAAC2160350.044.01559438-39
ATAACCG2181400.043.65073440-41
AGCGATA2189250.043.38689836-37
CGGAGTA2241100.041.8696744-45
TAAGACG2327500.041.01534364-65
GAGTCGC2304650.040.94781528-29
Produced by FastQC (version 0.11.5)