FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175659225
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAACATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT23639391.3457528347856482No Hit
GGCCGGAACATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA8633040.4914652219375327No Hit
GGCCGGAACATGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2534370.1442776489535349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA4384100.086.392474
CCGGAAC4459450.084.964753
GCCGGAA4480550.084.530262
GGCCGGA4601800.082.354941
GGAACAT4894900.077.234465
CATGGCG3464350.072.645699
GAACATG4230250.068.450976
ACATGGC4108450.061.407028
AACATGG4982650.050.8393367
AACATTG1764150.050.7524767
GAACATT1823100.049.2718126
ACATTGA1857450.048.1290328
CATTGAA1881100.047.4628529
GCGATAA2855100.044.396936-37
CGATAAC2859700.043.9222438-39
ATAACCG2874000.043.7301340-41
AGCGATA2885900.043.5456736-37
CGGAGTA2888450.042.24423644-45
AGTCGCC2972600.041.80176528-29
GAGTCGC2955150.041.74752828-29
Produced by FastQC (version 0.11.5)