FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121696122
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACATTGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT43797673.598937195385733No Hit
CTGCACATTGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA14219611.1684521878190992No Hit
CTGCACATTGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2702790.22209335479071385No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA2652120.21792970527031255No Hit
CTGCACATTGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2461660.20227924764932115No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT1772640.14566117398547837No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG1734320.14251234727101655No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT1580250.1298521246223442No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT1521540.12502781312949315No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC1513520.12436879459478585No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTG1452870.11938506964092085No Hit
CGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA1417720.1164967278086314No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG1406970.11561338002208485No Hit
CGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTC1338980.11002651341675455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACATT7075200.089.221223
ACATTGG7111150.088.692185
CACATTG7198950.087.6899644
TGCACAT7258050.087.072512
CATTGGG5665300.086.541826
ATTGGGG5063900.086.514777
TGGGGCG5337550.082.0062949
CTGCACA7790150.081.233891
TTGGGGC5414900.080.9199458
ATTGGTG1863600.075.4202047
CATTGGT1873750.075.0420766
ATTGGGT756800.068.575867
TTGGTGA2233400.062.9280858
TTGGGTA830500.062.084278
TGGGTAC903350.057.1301089
TGGTGAA2855700.049.2833029
ATCTCGA5123100.043.04677618-19
GCGATAA5135600.042.8628136-37
CGTCCAT5125150.042.82077814-15
AGCGATA5143750.042.5815936-37
Produced by FastQC (version 0.11.5)