FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121696122
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACATTGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT47636553.9143852094153013No Hit
CTGCACATTGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA16141411.3263701204874876No Hit
CTGCACATTGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC2514650.2066335359478423No Hit
CTGCACATTGGGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC2421340.19896607716061815No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA2385620.19603089735266993No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT1693770.13918027724827584No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC1306360.10734606645888027No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT1302120.10699765765748888No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA1273090.10461220777437756No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT1223580.10054387764303616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACATT8053400.090.0701143
ACATTGG8135750.088.933915
CACATTG8198000.088.290254
TGCACAT8235300.088.1812742
CATTGGG6415900.086.78916
ATTGGGG5812150.086.633847
CTGCACA8711050.083.618721
TGGGGCG6120800.082.3633969
TTGGGGC6156800.081.7201468
CATTGGT2156300.077.1488346
ATTGGTG2164550.076.8481147
ATTGGGT785100.068.169737
TTGGTGA2528900.065.670258
TTGGGTA866250.061.8124478
TGGGTAC933750.057.3885159
TGGTGAA3214250.051.919939
CGTCCAT5893700.042.90129514-15
ATCTCGA5946600.042.68544418-19
GGGGCGT5921050.042.55188410-11
GCGTCCA6014900.042.29894312-13
Produced by FastQC (version 0.11.5)