FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131089764
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTCGATATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT8936290.6816924317599656No Hit
GTAGTCGATATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2625170.2002574358132188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA1532450.087.841992
GTCGATA1531700.087.584174
GTAGTCG1542300.087.355911
AGTCGAT1590300.084.482873
CGATATG1271350.084.404736
TCGATAT1592600.084.199225
TATGGCG1197200.081.025199
CGATATT422400.064.030876
ATATGGC1525500.063.8494228
GATATGG1564800.062.418847
GCGATAA1183900.042.3649236-37
CGATAAC1182300.041.1750138-39
ATAACCG1195300.040.77306740-41
AGCGATA1201100.040.51459536-37
TAACCGG1250050.040.20889740-41
CGGAGTA1224100.040.0918144-45
TAAGACG1277850.039.37720564-65
AGTCGCC1262100.039.32900628-29
GAGTCGC1257150.038.4260228-29
CGACCAA1310300.037.26195570-71
Produced by FastQC (version 0.11.5)