FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108252708
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAATGGCGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT6552710.6053160351425111No Hit
CATAATGGCGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1881190.17377763889287648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGGC1336100.069.959523
CATAATG1344200.069.739151
ATAATGG1342900.069.686632
ATGGCGA1346550.069.201435
TGGCGAG1147650.064.2490166
AATGGCG1527350.061.092014
GCGAGGC1161850.057.09688
GGCGAGG1326350.050.1968047
CGAGGCG1462800.045.346719
CGATGAA444650.044.2586339
GCGATGA458500.042.890638
GCGATAA780750.042.72786336-37
CGATAAC782850.042.2856438-39
ATAACCG788400.042.04899240-41
AGCGATA803450.041.2044136-37
TGGCGAT485500.040.693076
TAACCGG838300.039.83494640-41
TAAGACG842050.039.3152164-65
CGGAGTA843900.038.82127844-45
GCGAGTA194550.038.6163988
Produced by FastQC (version 0.11.5)