FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89046603
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGATTCGTGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1833110.20585962161858098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG320600.084.109782
GATTCGT356250.075.769613
TCGTGCG300750.069.731046
ATTCGTG387550.069.622894
CCGATTC398550.067.7291261
TTCGTGC456450.059.304055
CGTGCGG386100.048.7815557
TGCGGCG468000.040.612879
GTGCGGC526500.036.143428
GCGATAA267100.036.0040636-37
CGATAAC264650.035.5645538-39
ATAACCG263500.035.48851440-41
TAACCGG286300.033.400540-41
AGCGATA283750.033.25525336-37
TAAGACG300000.031.42135464-65
CGGAGTA315650.029.41447444-45
CGACCAA317700.029.3829870-71
AGTCGCC359550.026.48882128-29
GAGTCGC355800.026.22076428-29
AGACGAC365600.025.94587566-67
Produced by FastQC (version 0.11.5)