FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112220769
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2517880.22436845001480968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG595500.057.3220564
TCAGCGC664300.051.435395
AGCGCGG485450.050.8702667
CAGCGCG562800.049.8687936
CGCGGCG564300.043.97359
GCGCGGC611400.040.6860168
GCGATAA338000.036.60370636-37
CGATAAC340150.036.1627338-39
ATAACCG345950.035.6660340-41
AGCGATA361250.034.1624436-37
TAACCGG364250.034.08929440-41
TAAGACG382250.032.40318364-65
CGGAGTA386850.031.58127844-45
TGCATCA1120750.030.753151
CGACCAA410500.030.07189270-71
GCATCAG1178000.029.2483062
GAGTCGC425450.029.14828528-29
GCGGCGT439300.028.32447610-11
AGTCGCC441950.028.1567728-29
GCGCGTA121100.028.0785068
Produced by FastQC (version 0.11.5)