FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112220769
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2816060.2509392891435274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG653450.058.7219164
TCAGCGC729300.052.7596935
AGCGCGG563500.051.3976027
CAGCGCG633950.050.5879756
CGCGGCG625500.046.279539
GCGCGGC681900.042.7031948
GCGATAA385100.037.5221836-37
CGATAAC386250.036.87698438-39
ATAACCG393750.036.23990640-41
AGCGATA408900.035.0884836-37
TAACCGG413600.034.8680940-41
TAAGACG433650.033.5214164-65
TGCATCA1162550.033.2577551
CGGAGTA432950.032.7062244-45
GCATCAG1225000.031.5809462
CGACCAA458650.031.4265670-71
GAGTCGC477550.030.0555628-29
AGTCGCC484550.029.97919328-29
GCGGCGT491400.029.4325110-11
AACCGGA504900.028.5065442-43
Produced by FastQC (version 0.11.5)