FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114495947
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGCAGTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT2430850.21230882521981326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC381950.081.901712
ATATACG412650.075.989061
ATACGCA438100.071.4479753
TACGCAG468800.066.834694
ACGCAGT522900.059.995965
GCGATAA335900.035.68180536-37
ATAACCG340650.034.88484640-41
CGATAAC340950.034.8065938-39
TAACCGG354850.033.89056840-41
AGCGATA362450.032.83873736-37
CGCAGTG819150.032.2299846
TAAGACG384500.031.14589964-65
CGGAGTA389000.030.5966244-45
CGACCAA407600.029.21470-71
AGTGGCG828550.029.2113599
GAGTCGC410400.028.99836328-29
AGTCGCC424500.028.46596128-29
AACCGGA433400.027.68548242-43
AGACGAC451050.026.70403166-67
ACCGGAG474550.025.53010742-43
Produced by FastQC (version 0.11.5)