FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005729612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96422866
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTAAGAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT7433530.7709302065342052No Hit
GGCTTAAGAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2237330.23203313620650937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG1311650.076.960842
GGCTTAA1320500.076.420631
CTTAAGA1441000.070.692053
TTAAGAG1515050.067.270774
TAAGAGA1580200.064.482245
AGAGGCG1294750.057.100869
AAGAGAG1825900.044.0797846
GCGATAA892950.041.91361636-37
CGATAAC894300.041.2030738-39
GAGAGGC1801250.041.173668
ATAACCG903550.040.845240-41
AGCGATA912000.040.33505236-37
TAACCGG949950.039.5851340-41
CGGAGTA953000.038.9444544-45
AGAGAGG1921000.038.745457
TAAGACG972000.038.58493464-65
ATCTCGA1010650.037.2348418-19
AGTCGCC1011800.036.90743328-29
GAGTCGC1007100.036.4665928-29
CGACCAA1010850.036.397570-71
Produced by FastQC (version 0.11.5)