FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122451335
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAACATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5264550.4299299799385609No Hit
GGCCGGAACATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1264730.10328429657381849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA1009900.070.205514
GCCGGAA1059500.066.994962
CCGGAAC1070150.066.319483
GGCCGGA1224100.058.2341731
GGAACAT1439800.049.6073045
CATGGCG1109500.048.266639
GCGATAA663600.040.4658136-37
CGATAAC661900.040.23962438-39
ATAACCG672850.039.69780340-41
GAACATG1499950.039.3818936
AGCGATA690300.038.5841236-37
TAACCGG700300.038.42327540-41
CGGAGTA726500.036.33156244-45
TAAGACG739700.036.25437564-65
CGACCAA781850.034.0470170-71
ACATGGC1589950.033.971228
GAGTCGC781400.033.78200528-29
AGTCGCC796100.033.48935328-29
AACCGGA801700.033.362142-43
ATCTCGA813750.033.24115418-19
Produced by FastQC (version 0.11.5)