FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112599239
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAACATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT5596360.4970157924424339No Hit
GGCCGGAACATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2119430.1882277374894159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA1175950.075.294834
CCGGAAC1225700.072.2372743
GCCGGAA1230150.071.918082
GGCCGGA1385950.064.0095751
GGAACAT1546450.057.524345
CATGGCG1137100.050.924449
GAACATG1509350.045.2437176
GCGATAA684750.042.1910136-37
CGATAAC684700.041.8395138-39
ATAACCG693350.041.3240240-41
AGCGATA709300.040.4327236-37
TAACCGG747250.038.6356540-41
TAAGACG740200.038.4763564-65
CGGAGTA734900.038.03039644-45
ACATGGC1599350.036.568378
CGACCAA772300.036.27327770-71
GAGTCGC804000.035.6938328-29
AGTCGCC814950.035.4269628-29
ATCTCGA836450.034.90875618-19
AGACGAC826000.034.32391766-67
Produced by FastQC (version 0.11.5)