FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112599239
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCGGAACATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT9772350.8678877483354928No Hit
GGCCGGAACATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1916940.17024448984064625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACA1604150.080.0594564
CCGGAAC1660700.077.32283
GCCGGAA1667350.077.047482
GGCCGGA1793300.071.7559741
GGAACAT1962150.065.687935
CATGGCG1590700.063.45389
GAACATG1935950.056.516496
ACATGGC2051450.049.4278838
GCGATAA1163250.043.48631736-37
CGATAAC1162600.043.11525338-39
ATAACCG1170750.042.8351340-41
AGCGATA1185800.042.36499836-37
TAACCGG1232300.041.0617440-41
CGGAGTA1218450.040.6932544-45
TAAGACG1243600.040.5938664-65
AGTCGCC1268800.039.48969728-29
GAGTCGC1264550.039.38207228-29
CGACCAA1275900.039.01177270-71
ATCTCGA1301300.038.9384818-19
AACATGG2636200.038.7782337
Produced by FastQC (version 0.11.5)