FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106800353
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTGTGGCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT3066960.2871675901670475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTGT1123350.041.0922931
GGTTGTG1149350.040.0298422
TTGTGGC1158750.039.495994
GTTGTGG1177750.038.955643
GCGATAA408950.038.35426736-37
CGATAAC414750.037.26663238-39
ATAACCG421200.036.6344840-41
AGCGATA434400.035.7245636-37
GCCGGCG883600.035.2737129
TAACCGG453300.034.52219440-41
TAAGACG475150.032.87036564-65
CGGAGTA483600.032.12389844-45
CGACCAA502300.030.80874370-71
GTGGCCG1156400.030.4801246
TTCGCGT188400.028.77591918-19
GAGTCGC541200.028.76172428-29
AACCGGA540800.028.71295242-43
AGTCGCC551000.028.64235728-29
ATCTCGA556050.028.42520918-19
AGACGAC561000.028.06405366-67
Produced by FastQC (version 0.11.5)