FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106800353
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTTGTGGCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT4404200.4123769141474654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTGT1196400.046.3678781
GGTTGTG1201300.046.1225052
GTTGTGG1240450.044.585143
TTGTGGC1253100.044.032634
GCCGGCG1051450.042.5916259
GCGATAA596450.037.86136-37
GTGGCCG1277850.037.7216766
CGATAAC606750.036.566138-39
ATAACCG609700.036.43234640-41
AGCGATA620050.035.82253336-37
TAACCGG637850.035.47228240-41
GGCCGGC1285600.035.0007638
TGGCCGG1319250.034.1260077
CGGAGTA684350.032.8614344-45
TAAGACG700800.032.1797364-65
AGTCGCC716250.031.43752928-29
GAGTCGC715300.030.98798828-29
CCGGCGT722650.030.876810-11
ATCTCGA747500.030.36628318-19
GATAACC760650.029.94514338-39
Produced by FastQC (version 0.11.5)