FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125083821
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCACTTAATGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT11298200.9032503092466291No Hit
TTGCACTTAATTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA2665830.21312348621009905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTTA2043400.073.5756763
ACTTAAT2076000.072.227415
CACTTAA2102700.071.26734
CTTAATG1826850.066.916076
TAATGGC1747800.065.786418
TTGCACT2355650.063.732051
TGCACTT2424250.062.2365382
TTAATGG1850700.062.222537
AATGGCG1936550.059.360079
GCGATAA1451150.041.8727936-37
TAACCGG1519200.039.83016240-41
CGATAAC1453150.039.711238-39
ATAACCG1459550.039.4337740-41
CGGAGTA1521150.039.3473444-45
AGCGATA1478150.039.0818436-37
AGTCGCC1547800.038.55525228-29
TAATTGA737000.038.1274458
TAAGACG1569250.038.1224364-65
ATCTCGA1599000.038.0414218-19
GATAACC1632600.037.16613838-39
Produced by FastQC (version 0.11.5)