FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005729624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005729624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109355749
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTGATACTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT12772401.1679678587359865No Hit
ATAGTGATACTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA1830730.16741049434904423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATAC1850850.081.205424
TAGTGAT1905350.078.831912
TGATACT1936450.077.601045
AGTGATA1937750.077.504913
ATAGTGA2013250.074.5754551
GATACTG1812150.073.2377556
ATACTGG1820400.070.409137
TACTGGC1830100.069.968478
ACTGGCG2589400.049.9061939
GCGATAA1477550.043.78414536-37
CGATAAC1480650.043.3888238-39
ATAACCG1491050.043.1167140-41
AGCGATA1504150.042.6877536-37
TAACCGG1534950.042.22550240-41
CGGAGTA1537850.041.64724344-45
TAAGACG1554900.041.19585464-65
ATCTCGA1608100.040.2348618-19
AGTCGCC1602150.040.05166228-29
GAGTCGC1599600.039.96109828-29
GATAACC1632250.039.76491538-39
Produced by FastQC (version 0.11.5)